Develop genetic therapies with us

The Nanogene Network, based in Minas Gerais, aims to develop innovative vaccines and gene therapies using advanced nanocarrier technology, integrating six leading research institutions.

View our Projects

Explore our Tools

The network is composed of six leading research institutions:

Our Key Objectives

Molecular target prediction

Nanocarrier characterization

Vaccine application

Gene therapy application

Scalability

Genetic material stabilization

Delivery enhancement

In vitro safety evaluation

In vivo efficacy studies

Tool creation

Methodology Overview

Multiple platforms drive the Nanogene Network’s research, each tailored to specific therapeutic goals.

1. Prediction of Molecular Targets

Selection of target genes from human genome databases and construction of RNA double-strand sequences encoding key disease-related proteins. Structural validation using X-ray crystallography and computational modeling.

2. Development and Characterization of Nanocarriers

Lipid nanoparticles (LNPs) synthesized through lipid solubilization, film hydration, and sonication. Genetic material encapsulation, size, stability, and encapsulation efficiency characterization.

3. Application in Vaccines

Evaluation of LNPs for safety through in vitro assays measuring cell viability and in vivo studies assessing clinical outcomes and tissue histology. Immunogenicity studies determine immune response effectiveness against targeted diseases.

4. Application in Gene Therapy

Testing LNPs for cellular uptake and gene transfection efficacy using fluorescently labeled genetic material. Assessment of gene expression and distribution in tissues using advanced microscopy and flow cytometry techniques.

5. Scaling Up

Transitioning validated formulations to pilot-scale production with meticulous quality control and adherence to regulatory standards. Development of master formulas and production protocols for clinical trials and future commercialization.

6. Technology Development

Integration of artificial intelligence, machine learning, and data mining to optimize target selection, nanocarrier design, and therapeutic efficacy. Enhancing decision-making processes for rapid advancement in therapeutic development.

siRNA Seeker: A Design Tool

Change default variant when you need

Our siRNA Design Tool employs robust algorithms from Reynolds et al. (2004), Ui-Tei et al. (2004), and Amarzguioui et al., enabling precise and rapid identification of therapeutic siRNA based on endorsed methodologies.

Advanced Algorithms for Targeted siRNA Development

Utilize advanced features like RNA translation, customizable siRNA length, author-specific scoring, melting temperature (Tm) calculation, Gibbs free energy (ΔG°) estimation, and integrated BLAST analysis for precise gene identification.

Unlock the power of siRNA design

Enhance your research efficiency with a tool that combines cutting-edge algorithms with user-friendly design, empowering biomedical researchers to accelerate discoveries and applications in RNA interference technology.

Frequently asked questions

Can’t find the answer you’re looking for? Reach out to our customer support team.

Is the siRNA Design Tool available for academic use?

Absolutely! Our siRNA Design Tool is freely available for academic purposes. We encourage its use in various research and therapeutic applications.

How accurate are the algorithms used in the siRNA Design Tool?

The algorithms in our siRNA Design Tool are based on validated methodologies from prominent researchers in the field, ensuring high accuracy in identifying potential therapeutic siRNA.

Is there technical support available for the siRNA Design Tool?

No, technical support is not provided for the siRNA Design Tool at this time. However, the tool is designed to be user-friendly and intuitive, with detailed documentation available to assist users.